. 2018 May 30;13(5):e0198085. doi: 10.1371/journal.pone.0198085

Table 2. Primer sequences, target genes, and cycling conditions for SYBR green RT-PCR.

Target gene	Primer sequences (5’–3’)	Reverse transcription	Primary denaturation	Amplification (40 cycles)			Dissociation curve (1 cycle)			Product size (bp)	Accession no.	References
Target gene	Primer sequences (5’–3’)	Reverse transcription	Primary denaturation	Secondary denaturation	Annealing (optics on)	Extension	Secondary denaturation	Annealing	Final denaturation	Product size (bp)	Accession no.	References
β-actin	`F: CCACCGCAAATGCTTCTAAAC`	50°C 30 min	94°C 5 min	94°C 15 s	51°C 30 s	72°C 30 s	94°C 1 min	55°C 1 min	94°C 1 min	175	NM205518	[47]
β-actin	`R: AAGACTGCTGCTGACACCTTC`				51°C 30 s			55°C 1 min		175	NM205518	[47]
ACC	`F: AATGGCAGCTTTGGAGGTGT`				60.9°C 30 s			60.9°C 1 min		119	NM_205505	[48]
ACC	`R: TCTGTTTGGGTGGGAGGTG`				60.9°C 30 s			60.9°C 1 min		119	NM_205505
CPT1	`F: CAATGAGGTACTCCCTGAAA`				57.5°C 30 s			57.5°C 1 min		337	AY675193
CPT1	`R: CATTATTGGTCCACGCCCTC`				57.5°C 30 s			57.5°C 1 min		337	AY675193
PEPT1	`F: CCCCTGAGGAGGATCACTGTT`				60°C 30 s			60°C 1 min		205	NM_204365	[49]
PEPT1	`R: CAAAAGAGCAGCAGCAACGA`				60°C 30 s			60°C 1 min		205	NM_204365
GLUT2	`F: CACACTATGGGCGCATGCT`				60°C 30 s			60°C 1 min		116	NM_207178.1
GLUT2	`R: ATTGTCCCTGGAGGTGTTGGTG`				60°C 30 s			60°C 1 min		116	NM_207178.1

β-actin, Beta actin; ACC, acetyl-CoA carboxylase; CPT1, carnitine acyltransferase I; PEPT1, peptide transporter 1; GLUT2, glucose transporter 2.