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. 2018 May 14;12(5):e0006450. doi: 10.1371/journal.pntd.0006450

Fig 5. VSG biosynthesis in AN5568-treated cells.

Fig 5

A) Three metabolites, all known to be involved in glycoprotein biosynthesis, were found to be at higher levels in AN5568-treated cells. B) Western blots carried out on protein lysates taken from AN5568-treated cells at a 6-hour time-point showed no significant changes in VSG expression at this time-point. When the contrast of the resulting blot was increased, an extra band was seen in the 2× EC50-treated sample. C) In addition to VSG expression, blots were probed with a VSG cross-reacting determinant (VSG XR) antibody. For this experiment, protein lysates were fractionated to isolate the membrane fraction and separate it from the soluble fraction. Most of the cellular GPI is found in the membrane, and no changes in expression could be seen in cells treated with 10× EC50 for 6 hours. WT; untreated wild-type cells, AN5568; wild-type cells treated with AN5568 for 6 hours at 10× EC50.