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. Author manuscript; available in PMC: 2018 May 30.

Published in final edited form as: Adv Biosyst. 2017 Jul 20;1(9):1700057. doi: 10.1002/adbi.201700057

Expression of CD34 (an EPC marker) in ECs and EPCs as a function of time and substratum by FACS. A) Expression in MEECs and ME-EPCs at 3 d is as low as that of 2D-ECs and 2D-EPCs; however, expression increases in both cell types in 3D as a function of time. B) While mature ECs in the vessel wall have only ≈1% CD34+ cells,^[18] MEECs 14 d in culture have a CD34 expression that matches and even surpasses that of ME-EPCs. Conversely, EPCs are highly CD34-expressing in vivo,^[17] but lose this marker when cultured in 2D, resulting in expression levels that are comparable to 2D-ECs. n = 3; *p < 0.05.

Expression of CD34 (an EPC marker) in ECs and EPCs as a function of time and substratum by FACS. A) Expression in MEECs and ME-EPCs at 3 d is as low as that of 2D-ECs and 2D-EPCs; however, expression increases in both cell types in 3D as a function of time. B) While mature ECs in the vessel wall have only ≈1% CD34+ cells,^[18] MEECs 14 d in culture have a CD34 expression that matches and even surpasses that of ME-EPCs. Conversely, EPCs are highly CD34-expressing in vivo,^[17] but lose this marker when cultured in 2D, resulting in expression levels that are comparable to 2D-ECs. n = 3; *p < 0.05.