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. 2001 Sep 25;98(21):12038–12043. doi: 10.1073/pnas.201112398

Figure 1.

Figure 1

Characterization of the nuclear expressed luciferase-EGFP fusion protein. Hamster lung fibroblasts were transfected with plasmids encoding nuclear luciferase (N-luc), EGFP, or N-luc-EGFP. Forty-eight hours after transfection, the cells were lysed before (C), directly after a heat shock of 45°C for 30 min (H), or after 3 h of recovery at 37°C (R). Cycloheximide (20 μg/ml) was added to the cells to prevent novel protein synthesis. (A) Western analysis with an antibody to luciferase of lysates of cells expressing N-luc or N-luc-EGFP. (B) Western analysis with an antibody to EGFP of total cell lysates (L) and detergent-soluble (S) and detergent-insoluble fractions (I) of EGFP- or N-luc-EGFP-transfected cells (lanes 1–3) or with an antibody to luciferase (lanes 4–6). (C) Confocal microscopy pictures of the subnuclear distribution of EGFP before (control), immediately after (30′ 45°C), and 3 h after (+3h 37°C) heat shock.

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