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. 2001 Sep 25;98(21):12038–12043. doi: 10.1073/pnas.201112398

Table 1.

Quantification of fluorescent staining patterns of N-luc-EGFP: Effects of Hsp70 and Hsp70(1–543) expression

Cells Treatment Immunofluorescent staining pattern, %
Luciferase activity
Diffuse Only small foci Small foci > nucleolar Small foci-nucleolar Small foci < nucleolar Only nucleolar
Control Unheated 96 4 100%
30 min at 45°C 8 35 49 9 0.7%  ± 0.1
+3 h at 37°C 6 58 30 6 11%  ± 0.7
Hsp70* Unheated 98 2 100%
30 min at 45°C 32 35 25 5 2%  ± 0.3
+3 h at 37°C 38 4 18 20 4 20 25%  ± 1
Hsp70(1–543)* Unheated 94 6 100%
30 min at 45°C 33 49 16 0.7%  ± 0.1
+3 h at 37°C 6 25 47 17 5 9%  ± 1.1
*

Only cells in which Hsp70 expression was detected by immunofluorescence were judged for these conditions. 

For each condition of each cell population, 45–55 cells were judged by taking confocal microscopy images. 

Luciferase activity was measured in the parallel experiment as described in the Materials and Methods and in Fig. 2 (mean ± SD). 

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