Table 1.
Quantification of fluorescent staining patterns of N-luc-EGFP: Effects of Hsp70 and Hsp70(1–543) expression
Cells | Treatment† | Immunofluorescent staining pattern, %
|
Luciferase activity‡ | |||||
---|---|---|---|---|---|---|---|---|
Diffuse | Only small foci | Small foci > nucleolar | Small foci-nucleolar | Small foci < nucleolar | Only nucleolar | |||
Control | Unheated | 96 | 4 | 100% | ||||
30 min at 45°C | 8 | 35 | 49 | 9 | 0.7% ± 0.1 | |||
+3 h at 37°C | 6 | 58 | 30 | 6 | 11% ± 0.7 | |||
Hsp70* | Unheated | 98 | 2 | 100% | ||||
30 min at 45°C | 32 | 35 | 25 | 5 | 2% ± 0.3 | |||
+3 h at 37°C | 38 | 4 | 18 | 20 | 4 | 20 | 25% ± 1 | |
Hsp70(1–543)* | Unheated | 94 | 6 | 100% | ||||
30 min at 45°C | 33 | 49 | 16 | 0.7% ± 0.1 | ||||
+3 h at 37°C | 6 | 25 | 47 | 17 | 5 | 9% ± 1.1 |
Only cells in which Hsp70 expression was detected by immunofluorescence were judged for these conditions.
For each condition of each cell population, 45–55 cells were judged by taking confocal microscopy images.
Luciferase activity was measured in the parallel experiment as described in the Materials and Methods and in Fig. 2 (mean ± SD).