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. 2001 Oct 2;98(21):12044–12049. doi: 10.1073/pnas.221430898

Figure 2.

Figure 2

ATR and BRCA1 are required for ICRF-193-induced mitotic delay. (A) Uninduced or induced GM847 cells were irradiated with 1.0 Gy of γ-rays (IR) or incubated with 2 μM ICRF-193 (I). Sham-treated and DMSO controls are denoted S and D, respectively. After 2 h, cells were harvested and Western immunoblot analysis was performed. (B) Uninduced (open bars) and induced GM847 (closed bars) cells were treated as described above. After 2 h, cells were assayed for mitosis. The results are expressed as the mean percentage of the untreated control (+ SD, for ICRF-193, n = 3; for IR, n = 2). *, The mean inhibition of mitosis in uninduced ICRF-193-treated cells was significantly different from that in induced ICRF-193-treated cells (Student's t test, P < 0.005). (C and D) Metaphase spreads of chromosomes from ATRki-induced GM847 cells. Arrows point to various chromosomal aberrations. (E) HCC1937 cells expressing GFP or BRCA1wt were irradiated with 1.0 Gy of γ-rays or incubated with 2 μM ICRF-193. After 2 h, cells were assayed for mitosis. The results are expressed as the mean percentage of the untreated control (+ SD, for ICRF-193, n = 3; for IR, n = 2). *, The mean inhibition of mitosis in GFP- expressing cells was significantly different from that in BRCA1wt-expressing cells (Student's t test, P < 0.005).