Key resources table.
| Reagent type | Reagent | Source | Cat. no. | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | RO-3306 | R and D Systems, Minneapolis, MN | 4181 | |
| Chemical compound, drug | Ciliobrevin D | MilliporeSigma, Burlington, MA | 250401 | |
| Chemical compound, drug | Nocodazole | Sigma-Aldrich, St. Louis, MO | M1404 | |
| Chemical compound, drug | Cytochalasin B | ACROS Organics, Geel, Belgium | 228090250 | |
| Chemical compound, drug | Cycloheximide | Alfa Aesar, Tewksbury, MA | J66901 | |
| Chemical compound, drug | Emetine | MilliporeSigma, Burlington, MA | 324693 | |
| Chemical compound, drug | Puromycin | MilliporeSigma, Burlington, MA | 540222 | |
| Chemical compound, drug | Harringtonine | LKT Laboratories, St. Paul, MN | H0169 | |
| Antibody | Rabbit anti-PCNT N terminus | Abcam, Cambridge, MA | Abcam Cat# ab4448, RRID:AB_304461 | 1:500 or 1:1000 dilution |
| Antibody | Goat anti-PCNT C terminus | Santa Cruz Biotechnology Inc., Santa Cruz, CA | Santa Cruz Biotechnology Cat# sc-28145, RRID:AB_2160666 | 1:500 dilution |
| Antibody | Mouse anti-γ-tubulin | Sigma-Aldrich, St. Louis, MO | Sigma-Aldrich Cat# T6557, RRID:AB_477584 | 1:1000 dilution |
| Antibody | Rabbit anti-phospho-Histone H3(Ser10) | MilliporeSigma, Burlington, MA | MilliporeSigma Cat# 06–570, RRID:AB_310177 | 1:500 dilution |
| Antibody | Mouse anti-ribosomal protein S6 | Santa Cruz Biotechnology Inc., Santa Cruz, CA | Santa Cruz Biotechnology Cat# sc-28145, RRID:AB_1129205 | 1:500 dilution |
| Antibody | Sheep anti-digoxigenin-alkaline phosphatase | Roche Diagnostics, Mannheim, Germany | Roche Cat# 11093274910, RRID:AB_514497 | 1:5000 dilution |
| Antibody | Sheep anti-digoxigenin-peroxidase | Roche Diagnostics, Mannheim, Germany | Roche Cat# 11207733910, RRID:AB_514500 | 1:500 dilution |
| Commercial assay or kit | MEGAshortscript T7 kit | Thermo Fisher Scientific, Waltham, MA | AM1354 | |
| Commercial assay or kit | mMESSAGE mMACHINE T3 kit | Thermo Fisher Scientific, Waltham, MA | AM1348 | |
| Commercial assay or kit | Click-iT EdU Imaging Kit | Life Technologies, Carlsbad, CA | C10337 | |
| Model organism | Wild-type NHGRI-1 fish | A gift from Shawn Burgess, NHGRI/NIH, Bethesda, MA | ZIRC Cat# ZL12751, RRID:ZIRC_ZL12751 | |
| Model organism | pcnttup2 fish | This study | ||
| Model organism | pcnttup5 fish | This study | ||
| Cell line | HeLa cells | ATCC CCL-2. A gift from Susan Wente, Vanderbilt University, Nashville, TN | ATCC Cat# CCL-2, RRID:CVCL_0030 | |
| Cell line | RPE-1 cells | A gift from Irina Kaverina, Vanderbilt University, Nashville, TN | ATCC Cat# CRL-4000, RRID:CVCL_4388 | |
| Cell line | HeLa cells stably expressing scFv-sfGFP-GB1 and NLS-tdPCP-tdTomato | A gift from Xiaowei Zhuang, Howard Hughes Medical Institute, Harvard University, Cambridge, MA | ||
| Cell line | RPE-1 cells expressing Centrin-GFP | A gift from Alexey Khodjakov, Wadsworth Center, 485 New York State Department of Health, Rensselaer Polytechnic Institute, Albany, NY | ||
| Software | Huygens Professional | Scientific Volume Imaging b.v., Hilversum, Netherlands | Huygens Software, RRID:SCR_014237 | |
| Software | Imaris | Bitplane, Belfast, UK | Imaris, RRID:SCR_007370 | |
| Software | MATLAB | MathWorks, Natick, MA | MATLAB, RRID:SCR_001622 | |
| Software | Prism 7 | GraphPad, CA | Graphpad Prism, RRID:SCR_002798 |