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. 2018 Jan 26;2:PO.17.00091. doi: 10.1200/PO.17.00091

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© 2018 by American Society of Clinical Oncology

Licensed under the Creative Commons Attribution 4.0 License:https://creativecommons.org/licenses/by/4.0/

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Fig 1. — Workflows of RNA-seq library preparation. Red indicates steps only applied to fresh frozen (FF) samples, and blue indicates steps only applied to formalin-fixed paraffin-embedded (FFPE) samples. (*) Indicates different ribosomal RNA (rRNA) depletion methods that result in two different TotalRNA protocols, that is, RiboZero for I.TotalRNA and RNase H for K.TotalRNA protocol. CR, coding region capture protocol; deM, demodification protocol; mRNA, mRNA-targeting protocol; PCR, polymerase chain reaction; sRNA, sense RNA protocol.