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. 2018 Jan 26;2:PO.17.00091. doi: 10.1200/PO.17.00091

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© 2018 by American Society of Clinical Oncology

Licensed under the Creative Commons Attribution 4.0 License:https://creativecommons.org/licenses/by/4.0/

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Fig 2. — Scatter plot of the first three principal components for count per million–normalized and variance stabilizing transformed counts of 20,381 coding region (CR)-targeted poly(A)⁺ genes. Each point corresponds to one of 90 libraries. (A) Blue circles indicate samples prepared with CR and gold circles indicate those without CR treatment; 26.5% of total variation comes from CR treatment. (B) Blue color indicates fresh frozen (FF) samples and gold for formalin-fixed paraffin-embedded (FFPE) samples. The symbol shape indicates the different biologic group. The biologic differences and FFPE effects are captured, which accounts for 20.6% of total variation. ER, estrogen receptor; HER2, human epidermal growth factor receptor 2; PC, principal component; PR, progesterone receptor; W CR, with coding region capture; W/O CR, without coding region capture.