Figure 3. Detection of cell-surface and circulating GPC-1 isoforms by MIL-38 and 3G5.
Western blots of immuno-precipitates from cell membrane protein extracts, plasma, serum, and Buffer SB (MIL-38-conjugated Dynabead® negative control). Precision Plus Protein Western C standard served as the protein marker. (A) GPC-1 isoforms detected using MIL-38 primary antibody; blot imaged with a 30 sec exposure. (B) GPC-1 isoforms detected using 3G5 primary antibody; blot imaged with a 2 min exposure.