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. 2018 Apr 27;9(32):22359–22367. doi: 10.18632/oncotarget.25009

Figure 4. Recognition of soluble GPC-1 protein in cell culture medium using the MIL-38/3G5 Luminex® assay.

Figure 4

GPC-1 in RPMI-1640 medium without FBS from low GPC-1 expressing cells and high GPC-1 expressing cells (n = 3) was measured using the Luminex® assay with MIL-38 capture antibody and 3G5 detection antibody. GPC-1 concentrations (ng/mL) were normalized by cell density (106 cells/mL) to calculate soluble GPC-1 per 106 cells (mean ± SD). Statistical significance (***p = 0.0004) was determined by unpaired t-test.