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. 2018 Apr 27;9(32):22423–22435. doi: 10.18632/oncotarget.25051

Figure 4. SKI overexpression attenuates MYB dependent differentiation in AML cells.

Figure 4

(A) Expression of CD11b was analyzed by FACS in HL60 expressing alternative shRNAs (shMYB1, shMYB2) targeting MYB or a control shRNA (shCtrl) in absence or presence of SKI overexpression (SKI OE). The empty vector (mock) served as negative control for SKI overexpression. Expression of shRNAs was induced by doxycycline (dox) and cells were measured via FACS (anti-human CD11b-PE) 48 h later. Bar graphs show mean values ± s.d. of % CD11b-positive HL60 of a representative experiment performed in technical triplicates from four independent experiments with similar results. (B) Data with doxycycline induction are shown as one typical result from four independent experiments with similar results. The experiment was performed and measured as described in (A). (C) Representative Western Blot analyses of the experiments performed in (A) and (B) showing SKI overexpression and MYB knockdown by shRNAs in the doxycycline-inducible HL60 cells.