Abnormal morphologies of potential equine zygotes after intracytoplasmic sperm injection include (a) premature chromosome condensation, with female spindle (top left) and male chromosomes flanked by a spindle structure (lower right); (b) multiple pronuclei; (c) sperm chromatin induced ectopic polar body, represented by the two sets of chromosomes undergoing anaphase; (d) multipolar spindle; (e) scattered maternal chromosomes (blue, DNA; red, centromeres and kinetochores; green, tubulin).