Skip to main content
. Author manuscript; available in PMC: 2018 Jun 20.
Published in final edited form as: Mucosal Immunol. 2017 Dec 20;11(3):615–626. doi: 10.1038/mi.2017.99

Figure 1. Alternaria-induced pulmonary inflammation requires Pla2g5.

Figure 1

(A) Total cells counts from homogenate lungs of naive and Alternaria-treated Wt and Pla2g5-null mice. Analysis by flow cytometry of lung cell from naive and Alternaria-treated Wt and Pla2g5-null lung homogenates of (B) eosinophils gated as CD45+ CD11c− SiglecF+; (C) ILC2s gated as CD45+ Lin− Thy1.2+; (D) histograms of Sca-1, ST2, ICOS, CD25 gated on Thy1.2+ cells (isotype in gray, Wt in blue, Pla2g5-null in red), and (E) expression of intracellular IL-5 and IL-13 by Thy1.2+ ILC2s. Values are mean ± SEM of at least three independent experiments with 5–9 (naïve) or 10–21 (Alternaria-treated) mice per group. Images are from one representative mouse per group. *** P< 0.0005, **P< 0.005, *P< 0.05.