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. Author manuscript; available in PMC: 2018 Jun 20.
Published in final edited form as: Mucosal Immunol. 2017 Dec 20;11(3):615–626. doi: 10.1038/mi.2017.99

Figure 5. Intranasal administration of LA and OA in combination with r-IL-33 increased eosinophilia and ILC2 activation in Wt and Pla2g5-null mice.

Figure 5

(A) Production of FFAs measured by mass spectrometry in Wt and Pla2g5-null lung macrophages enriched by Percoll gradients of lung homogenates pulled from 5–8 mice. Flow cytometry analysis of (B, D) numbers of eosinophils and (C, E) Thy1.2+ ILC2s expressing intracellular IL-5 in homogenate lungs of Wt (black bars) and Pla2g5-null mice (white bars) treated i.n. with IL-33, LA or OA (B, C), or IL-33, LA, OA and AA (D, E) as indicated. Values are mean ± SEM of two independent experiments with 5 samples (A), two-four independent experiments (B, C, D) or one representative experiment (E) with 4–12 mice per group, and were compared by t-test (A) or One-way ANOVA with Sidak’s correction for multiple comparisons. **P< 0.005, *P< 0.05.