Skip to main content
. Author manuscript; available in PMC: 2018 Aug 7.
Published in final edited form as: Mucosal Immunol. 2018 Feb 7;11(3):752–762. doi: 10.1038/mi.2017.118

Figure 7. mTOR regulates butyrate induction of RegIIIγ and β-defensins in IEC.

Figure 7

MSIE cells were treated with 0.5 mM butyrate for 1 h. (A) Phosphorylation of mTOR and 4E-BP1 were determined by Western blot, with β-actin as a loading control. (B) The expression of phosphorylated S6K1 was determined by flow cytometry at 48 h post butyrate treatment. Combined median fluorescence intensity (MFI) was presented. (C and D) MSIE cells were transfected with mTOR siRNA or control non-targeting siRNA, followed by 0.5 mM butyrate for 48 h. (C) siRNA knockdown efficiency was confirmed by RT-PCR at 40 h post-transfection. (D) The expression of RegIIIγ and β-defensins were determined by qRT-PCR and normalized against gapdh 48 h post-treatment. *p<0.05; **p<0.01. Data are reflective of 3 independent experiments.