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. Author manuscript; available in PMC: 2018 Jul 20.
Published in final edited form as: ACS Chem Biol. 2017 Nov 30;13(7):1721–1733. doi: 10.1021/acschembio.7b00919

Figure 11.

Figure 11

O6-Benzylguanine labeled probes. The red portion of each molecule is transferred to a reactive cysteine residue in the active site of MGMT. (a) The dabcyl labeled O6-benzyl deoxyguanosine reported by Beharry and co-workers.126 The modified nucleoside is incorporated into fluorescently labeled oligonucleotides. MGMT activity removes the quenching dabcyl moiety. (b) The rotor based probe reported by Yu and co-workers.127 Upon transfer of the CCVJ moiety to MGMT, intramolecular bond rotation about the trisubstituted olefin is hindered causing CCVJ to become emissive.