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. Author manuscript; available in PMC: 2018 Jul 20.
Published in final edited form as: ACS Chem Biol. 2017 Nov 30;13(7):1721–1733. doi: 10.1021/acschembio.7b00919

Figure 6.

Figure 6

Example of a signal amplification probe of TDG reported by Chen et al.42 Upon repair of the G:T mismatch by TDG, the AP endonuclease EnIV cuts the DNA. The resulting 5′ terminal is digested by T7Exo. The G-containing template strand duplexes with the reporter oligonucleotide that is digested by T7 Exo to generate a fluorescent signal. Because the template strand is recycled, the signal of a single enzyme turnover is amplified. Reproduced from ref 42 with permission of The Royal Society of Chemistry, copyright 2013.