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. Author manuscript; available in PMC: 2018 Nov 14.
Published in final edited form as: Nat Immunol. 2018 May 14;19(6):571–582. doi: 10.1038/s41590-018-0107-1

Figure 6. LysM-AhR cKO mice spontaneously develop systemic autoimmunity.

Figure 6

(a) Serum samples from mice were examined for anti nuclear IgG antibody (ANA) reactivity by immunofluorescence staining. Scale bar=50μm. (b) ELISA for serum autoreactivity to dsDNA and histone in 62w-old mice. (c) Flow cytometry analysis of spleens from 62w-old female mice of the indicated genotype. (d) Kidneys were collected and 5μm paraffin sections were stained with H&E and pathologic alteration of the glomeruli and tubules was scored in a blinded manner. Scale bar=100μm. (e) Mouse kidney cryosections were stained for IgG, IgM, and complement C3. All images are representative of 5 mice/group. Graph data points represent individual mice and bars are the mean value/group. Scale bar=100μm. For a n=5 and for b and c n=10 individual mice per group and bars are the mean +/− standard deviation. **=P≤ 0.01, ***=P≤ 0.001, ***=P≤ 0.0001 as determined by two-sided Student’s t-test. All experiments were repeated twice with similar results.