Skip to main content
. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2018 Apr 22;1863(8):857–865. doi: 10.1016/j.bbalip.2018.04.015

Fig. 5. Myogenic differentiation is reduced in TAZ-KO cells.

Fig. 5

(A) Myogenic differentiation was initiated in cells reaching confluence by switching the cells to DMEM medium containing 2% horse serum. Cells were cultured for 1, 3, or 7 days. Antibody detection of skeletal muscle contractile protein myosin heavy chain (MHC) was used for immunofluorescent staining of differentiated myotubes. (B) Myotube density was quantified via morphometric analysis using ZenPro software (Zeiss). MHC-positive myotubes were manually traced, and the myotube density was calculated by dividing the area occupied by MHC-positive myotubes by the total area of the field of view (Data from at least three biological replicates were used for statistical comparisons, p<0.05).