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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Anal Biochem. 2018 Apr 23;550:84–89. doi: 10.1016/j.ab.2018.04.018

Figure 2.

Figure 2

Direct binding of UCH37C88A•RPN13 to five different fluorescein-labeled mono-Ub derivatives (Ub K6C-Fl, Ub K11C-Fl, Ub K29C-Fl, Ub K48C-Fl and Ub K63C-Fl). Data were fit to eq. 1 using nonlinear regression analysis, while holding the total fluorescent mono-Ub concentration constant and maintaining Q=0.8. Data reflect mean ± SEM of two independent experiments.