Skip to main content
. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Anal Biochem. 2018 Apr 23;550:84–89. doi: 10.1016/j.ab.2018.04.018

Figure 3.

Figure 3

Direct binding of Ub K48C-Fl to UCH37C88A•RPN13 (A) and UCH37C88A•RPN13DEUBAD (B). Data were fit to eq. 1 using nonlinear regression analysis, while holding the total fluorescent mono-Ub concentration constant and maintaining Q=1.1. Data reflect mean ± SEM of two independent experiments.