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Competitive displacement of Ub K48C-Fl from UCH37C88A•RPN13 using unlabeled mono-Ub, Lys48 Ub2, Ub3 and Ub4 (A). Competitive displacement of Ub K48C-Fl from UCH37C88A•RPN13DEUBAD using unlabeled monoUb, Lys48 Ub2, Ub3 and Ub4 (B). Data were fit to eq. 2 using nonlinear regression analysis, while holding the total fluorescent mono-Ub concentration constant. Data reflect mean ± SEM of two independent experiments.
