Figure 3.

Reduced Colitis Severity in ASL CKO Immune Cells

Colitis was induced by DSS in Vav1^Cre: Asl^f/f and in control Asl^f/f mice.

(A) A representative graph of endoscopic evaluation scores on day 12 after colitis induction, showing a significant improvement in the colitis score of the Vav1^Cre: Asl^f/f group compared with the control group. The experiment was performed 3 times with a total of n ≥ 20 mice in each group with 3 repetitions. Right: a representative image of the colon taken by colonoscopy.

(B) A representative experiment showing a significant increase in colon length in Vav1^Cre: Asl^f/f mice compared with the control group. The experiment was performed three times with a total of n ≥ 19 mice in each group.

(C) A T2 map of representative colon sections, demonstrating increased relaxation time in the control group compared with Vav1^Cre: Asl^f/f mice. Right: quantification of the images using Image-pro Pulse software. The experiment was performed on a total of n = 5 mice in each group.

(D and E) A significant reduction in histological score (D) and a lower mortality rate (E) in Vav1^Cre: Asl^f/f mice compared with controls.

(F) Anemia developed only in the control group after colitis induction, as shown by decreased red blood cells (RBCs), hemoglobin (HgB), and hematocrit (HCT) levels. No differences between groups were detected in the white blood cell (WBC) count (n = 3 in each group).

Error bars represent SEM.