Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 30;8:8412. doi: 10.1038/s41598-018-26840-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

3D xenoline microtumors as model of GBM. (A) Schematic for 3D microtumor production from PDX tumor cells. (B) Microtumor Calcein-AM live cell staining and IHC compared to PDX tumor. Microtumor characterization using Calcein-AM live cell staining (left 2 panels) and IHC staining of stem cell (CD-133) and proliferation (Ki-67) markers (middle two panels) is shown at low and high magnification to mimic in vivo tumors (H&E and Ki-67 staining shown for murine implanted tumors (right two panels) (magnification is indicated). (C) Selected PDX tumors with Verhaak et al. molecular subtype⁴⁰, median survival, EGFR, PTEN and TP53 status as well as Calcein-AM imaging of Microtumor beads in Neurobasal medium at Day 7 at 4x magnification and 250 ms exposure. Scale bar is 500 µm.