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. 2018 May 30;8:8398. doi: 10.1038/s41598-018-26371-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro colonization of acellular scaffold using MuSCs and FBs. (a) Representative scan images of scaffold cross-sections seeded with MuSCs, FBs or MuSCs-FBs analysed by immunofluorescence analysis using anti-laminin (red) and anti-MyHC antibodies (green). Laminin was used to identify the entire cross section of the scaffolds. Nuclei were stained with Hoechst (blue). Scale bar: 500 μm. (b) Representative images acquired at stereomicroscope of MTT assay performed on unseeded or seeded scaffolds cultured with MuSCs, FBs or MuSCs-FBs. Scale bar: 1 mm. (c) Analysis of cell distribution in scaffold cross-sections seeded with MuSCs or MuSCs-FBs. *P < 0.05, one-way ANOVA and Tukey’s multiple comparison test; (d) Availability assay by MTT quantification. Data are shown as mean ± s.e.m. of six independent replicates. **P < 0.01; one-way ANOVA and Tukey’s multiple comparison test.