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. 2018 May 24;9:1010. doi: 10.3389/fimmu.2018.01010

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Copyright © 2018 Khan, Alanazi, Ahmed, Vater, Assiri and Broering.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C5a blockade is associated with increased CD4⁺CD25⁺FOXP3⁺ regulatory T cell (Treg) numbers. Flow cytometry analysis of Tregs from peripheral blood of allograft recipients on d6, d10, and d14 posttransplantation: (A) Dot plot displaying SSC/FSC of lymphocytes, CD3⁺ positive CD4⁺ T lymphocytes, and isotype control analysis. (B) Percentage of CD4⁺FOXP3⁺ lymphocytes in peripheral blood. (C) Percentage of CD4⁺CD25⁺ lymphocytes in peripheral blood. (D) Ratio of CD4⁺FOXP3⁺ to CD4⁺ lymphocytes. (E) Percentage of CD4⁺CD25⁺FOXP3⁺ Tregs of peripheral lymphocytes. Data are presented as means with SE of 4–6 transplants/time point/experiment, and repeats of three different experiments. *p < 0.05.