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. 2018 May 24;9:1010. doi: 10.3389/fimmu.2018.01010

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Copyright © 2018 Khan, Alanazi, Ahmed, Vater, Assiri and Broering.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C5a blockade increased infiltration of regulatory T cell (Treg) in allograft. (A) Immunofluorescent staining for CD4 and forkhead box P3 (FOXP3) at d10 posttransplantation. (B–D) Morphometric analysis of graft infiltrating CD4⁺ T cells, FOXP3⁺ cells, and CD4⁺FOXP3⁺ colocalization (Tregs). Arrows highlighted individual CD4⁺ T cells (white arrows), FOXP3⁺ cells (blue arrows), and CD4⁺FOXP3⁺ Tregs (yellow arrows). Data are presented as means with SE of 3–4 transplants/time point/experiment, and repeats of three different experiments. *p < 0.05. Original magnification, ×40.