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. 2018 May 24;9:1152. doi: 10.3389/fimmu.2018.01152

Figure 1.

Figure 1

Gating strategy used to assess the identification and functional characterization of invariant natural killer T (iNKT) cells. (A) Initially, cells were gated on SSC and FSC followed by gate on live CD3+ T cells population (stained with violet amine reactive dye to identify live cells). The live CD3+ T cells further gated to identify iNKT cells as CD3+, Vα24+, and Vβ11+. (B) iNKT cells were further gated to assess the secretion of cytokines, such as IFN-γ, TNF-α, IL-2, IL-4, and/or IL-21 and CD107a expression at unstimulated and stimulated iNKT cells with α-GalCer (B1–12 respectively).