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. 2018 May 24;9:642. doi: 10.3389/fpls.2018.00642

FIGURE 2.

FIGURE 2

Interaction between GhCDKE and GhCycC. (A) Interaction between GhCDKE and GhCycC in yeast. GhCDKE was fused to the GAL4-binding domain (pGBKT7), and GhCycC was fused to the GAL4-activation domain (pGADT7). (B) Luciferase complementation imaging (LCI) assay of the interaction between GhCDKE and GhCycC in tobacco leaves. Agrobacterium tumefaciens GV3101 strains harboring constructs indicated on the left panel were transferred into tobacco leaves. LCI was performed 48 h after co-infiltration with the same amount of A. tumefaciens cells. Interaction between GhMLP28 and GhERF6 was used as the positive control.