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. 2018 May 24;9:1011. doi: 10.3389/fimmu.2018.01011

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Copyright © 2018 Ofir-Birin, Abou karam, Rudik, Giladi, Porat and Regev-Rudzki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization of double-stained Plasmodium falciparum (Pf)-derived extracellular vesicles (EVs) by imaging flow cytometry (IFC). (A) IFC analysis of double-stained Pf-derived EVs. Pf-derived EVs co-labeled with different combinations of two stains: for lipids (DiI, DiD, or DiO), RNA [thiazole orange (TO)], and proteins (GO). Representative results from at least three experiments are shown. Abbreviations: BF, bright field; SSC, side scatter (B) Uptake assay of Pf-derived EVs into monocytes (THP-1). IFC imaging of Pf-derived EVs labeled with two stains for lipids (DiI, DiD, or DiO) and/or RNA (TO) and/or proteins (GO) uptaken into THP-1 cells as described in the Section “Method.” Representative results from at least three experiments are shown. Abbreviations: BF, bright field; SSC, side scatter.