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Arabidopsis thaliana (ecotype Columbia-0) plants cultured hydroponically for 8 weeks with an 8-h photoperiod on nutrient medium containing 5 mM NO3– (NO3) or 5 mM NH4+ (NH4) as a sole nitrogen source. Plants grown on NH4+ showed severe growth retardation as compared to NO3–-grown plants. Leaf samples for assays were collected in the middle of the light period.
