Table 2.

Kinetics of the fermentations conducted with adapted and non-adapted Propionibacterium freudenreichii.

		Non-adapted P. freudenreichii				Adapted P. freudenreichii
		CCM$\frac{1}{2}$	CM$\frac{1}{2}$	CCM	CM	CCM$\frac{1}{2}$	CM$\frac{1}{2}$	CCM	CM
	Biomass (g L−1)	1.34 ± 0.01	1.35 ± 0.04ns	0.66 ± 0.02	0.84 ± 0.03*	1.53 ± 0.00	1.65 ± 0.02ns	1.14 ± 0.05	1.20 ± 0.02ns
	Growth rate (h−1)	0.10 ± 0.01	0.07 ± 0.00*	0.10 ± 0.00	0.04 ± 0.00***	0.11 ± 0.00	0.10 ± 0.01ns	0.13 ± 0.00	0.04 ± 0.00***
Acetate	Concentration (g L−1)	0.66 ± 0.01	1.11 ± 0.06**	0.53 ± 0.02	1.32 ± 0.02***	0.74 ± 0.00	1.07 ± 0.00***	0.62 ± 0.00	1.19 ± 0.02***
	Yield (g g−1)	0.24 ± 0.01	0.40 ± 0.02***	0.56 ± 0.01	1.26 ± 0.01***	0.23 ± 0.00	0.33 ± 0.00**	0.36 ± 0.02	0.66 ± 0.01***
	Productivity (g L−1 h−1)	0.01 ± 0.00	0.02 ± 0.00ns	0.009 ± 0.000	0.02 ± 0.00***	0.01 ± 0.00	0.02 ± 0.00ns	0.011 ± 0.001	0.02 ± 0.00***
Propionate	Concentration (g L−1)	2.21 ± 0.02	2.62 ± 0.06**	1.24 ± 0.05	2.48 ± 0.00***	2.66 ± 0.02	2.92 ± 0.07ns	1.84 ± 0.05	2.53 ± 0.03**
	Yield (g g−1)	0.78 ± 0.03	0.95 ± 0.02***	1.3 ± 0.0	2.36 ± 0.06***	0.82 ± 0.01	0.90 ± 0.02**	1.00 ± 0.01	1.40 ± 0.01***
	Productivity (g L−1 h−1)	0.03 ± 0.00	0.04 ± 0.00**	0.020 ± 0.001	0.04 ± 0.00***	0.04 ± 0.00	0.05 ± 0.00ns	0.03 ± 0.00	0.04 ± 0.00**

Colorectal cancer cells conditioned medium was used to culture the non-adapted and adapted bacteria. It consists of the supernatants collected after colorectal cancer cells growth in a DMEM medium without antibiotic for 72 h at 37°C in a humidified incubator with 5% CO₂. Glucose was the carbon source present in this medium. Each condition was run in duplicate and the results correspond to the mean ± standard error of the mean.

^*p < 0.05;

^**p < 0.01;

^***p < 0.001, values significantly different between conditioned medium conditions and respective controls; “ns” corresponds to non-significant differences. CM$\frac{1}{2}$, colorectal cancer cells conditioned medium diluted (1:1) in fresh DMEM; CCM$\frac{1}{2}$, control of diluted colorectal cancer cells conditioned medium; CM, Colorectal cancer cells conditioned medium; CCM, control of colorectal cancer cells conditioned medium.