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. 2018 May 24;10:159. doi: 10.3389/fnagi.2018.00159

FIGURE 2.

FIGURE 2

Nested PCR of fungal DNA from AD frozen tissue. PCR analysis was carried out as described (see section “Materials and Methods”). Schematic representation of fungal rRNA genes (18S, 5.8S and 28S rRNA) and the ITS-1 and ITS-2 regions, including location of the primers employed for the different nested PCRs: primers External 1 employed in the first PCR; primers Internal 1A and Internal 1B employed in the second PCR to amplify ITS-1; primers Internal 2 employed in the second PCR to amplify ITS-2 (A). Agarose gel electrophoresis of the DNA fragments amplified by nested PCR using DNA extracted from frozen FC tissue. PCR analysis of ten AD patients using primers Internal 1B to amplify the ITS-1 region (B). PCR analysis to amplify the ITS-2 region from 10 AD patients using primers Internal 2 (C). PCR analysis of DNA extracted from the samples tested in (B,C) using human β-globin oligonucleotide primers (D). Control–, PCR without DNA. CE, Control of DNA extraction without DNA. C+, DNA extracted from HeLa cells.