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. 2018 May 30;8:8413. doi: 10.1038/s41598-018-26563-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chemical characteristics of the female and mature male Calopteryx haemorrhoidalis wing membranes as determined by nanoprobe XRF. Microscopy image of the wing of the (A) female and (D) male damselfly with the scanned area marked as red square. Total fluorescence yield of the scanned area for the (B) female and (E) male damselfly wings. The blue area represent the wing membrane, while the red and green parts were obtained on the hair structure. Accumulated XRF spectra of the region of the hair (red) and of the wing (black) for (C) female and (F) male damselfly wings.