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. 2018 May 30;8:8393. doi: 10.1038/s41598-018-26717-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of clinical specimens by (A) LAMP-SNP and (B) virus isolation. (A) P: positive control (MEV SD), N: negative control (double distilled water), tubes 1–10 represent different clinical fecal samples. (B) Virus isolation (MEV-Z6) was identified by an indirect immunofluorescence assay using a monoclonal antibody against the VP2 protein of MEV and goat anti-mouse IgG-FITC conjugated secondary antibody. Specific immunofluorescent signals were detected in cells exposed to the respective tissue filtrates but not in mock-infected control cells. Full-length tubes and immunofluorescence pictures are presented in Supplementary Fig. 4A, B.