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. 2018 May 30;8:8362. doi: 10.1038/s41598-018-25382-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of genetic markers of diabetes in aging Drosophila melanogaster supplemented with the probiotic and synbiotic formulation. At 0, 10, 20 and 30 days, the expression of several insulin-signaling genes, namely (a) Drosophila insulin-like peptide (dilp)2, (b) dilp3, (c) insulin receptor (InR), (d) dAkt, (e) dTOR and (f) dFOXO. Each group contained n = 5 independent groups and significance is indicated as stars (*) relative to the control day 0 group with *p < 0.05 and **p < 0.01 and as tau (τ) as relative to the no-treatment control at the same time point where ^τp < 0.05.