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Assays for the phosphorylation of UvPmk1 and UvSlt2 MAP kinases. Western blots of total proteins isolated from the wild-type strain P1 and Uvslt2 mutant were detected with an anti-TpEY phosphorylation specific antibody and an anti- p44/42 antibody. Phosphorylation and expression of UvSlt2 was detected in the wild-type but not in the Uvslt2 mutant. Detection with an anti-H3 antibody (Cell Signaling Technology) was to show that similar amount of proteins were loaded for each sample.
