Table 1.
Strains and vectors used in this study.
| Strain | Brief description | Reference |
|---|---|---|
| P1 | Wild-type | Zheng et al., 2017 |
| CS-2 | Transformant of P1 expressing the pDHt/sk-PC | This study |
| MS-1 | Uvslt2 deletion mutant of P1 | This study |
| MS-2 | Uvslt2 deletion mutant of P1 | This study |
| MS-4 | Uvslt2 deletion mutant of P1 | This study |
| MS-5 | Uvslt2 deletion mutant of P1 | This study |
| MS-8 | Uvslt2 deletion mutant of P1 | This study |
| MS-9 | Uvslt2 deletion mutant of P1 | This study |
| MS-11 | Uvslt2 deletion mutant of P1 | This study |
| MS-12 | Uvslt2 deletion mutant of P1 | This study |
| MS-15 | Uvslt2 deletion mutant of P1 | This study |
| MU-45 | ustA deletion mutant of P1 | This study |
| MU-47 | ustA deletion mutant of P1 | This study |
| MU-49 | ustA deletion mutant of P1 | This study |
| MU-52 | ustA deletion mutant of P1 | This study |
| MU-54 | ustA deletion mutant of P1 | This study |
| MU-60 | ustA deletion mutant of P1 | This study |
| Vectors | ||
| pUC-H1-gRNA | Vector with the H1 promoter for gRNA expression | Zheng et al., 2014 |
| pDHt/sk-PC | pDH1/tk-Ppdc-toCas9-Tpdc | Liu et al., 2015 |
| pCRISPR/Cas-U6-1 | Cas9-gRNA vector with the U6 promoter | Arazoe et al., 2015 |
| pCBDW | Agrobacterium binary vector | Zheng et al., 2016 |
| pUC19-tRp-gRNA | Gln-tRNA promoter of gRNA | This study |
| pCAS9-tRp-gRNA | Cas9-gRNA vector with the tRNA promoter | This study |
| pCas9-tRp-UA01 | Cas9-gRNA vector with the UA01 spacer | This study |
| pCas9-tRp-UA13 | Cas9-gRNA vector with the UA13 spacer | This study |
| pCas9-tRp-UA21 | Cas9-gRNA vector with the UA21 spacer | This study |
| pCas9-tRp-SLT01 | Cas9-gRNA vector with the SLT01 spacer | This study |
| pHY2016A | pCAS9: tRp-SLT01 gRNA | This study |