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. 2018 May 30;8:8383. doi: 10.1038/s41598-018-26560-9

Figure 2.

Figure 2

Design of engineered CLDN-5 antigens for immunization. (a) Design of Antigen1. Alignment of the amino acid sequences among human CLDN-5, mouse CLDN-5 and human/mouse chimeric CLDN-5 (Antigen1) is shown. Putative TMDs are indicated by gray boxes. Asterisks indicate unmatched amino acids between human and mouse CLDN-5. (b) Schematic illustration of Antigen2. Red and blue colors indicate extracellular fragment 1 and 2, respectively. (c) Amino acid sequence of Antigen2. Putative TMDs are indicated by gray boxes. Red and blue font colors indicate extracellular fragment 1 and 2 of CLDN-5, respectively. (d) Cell-free synthesis of Antigen1 and Antigen2. Purified liposomes were subjected to SDS-PAGE and CBB staining.