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. 2017 Dec 1;3(3):555–572. doi: 10.1016/j.ekir.2017.11.019

Figure 3.

Figure 3

Quantitative polymerase chain reaction (qPCR) validation of microRNAs (miRNAs) from small-RNA sequencing of patients with type 1 diabetes (T1D). (a) qPCR results for miR-144-3p, miR-26a-5p, and miR-30c-5p in, urine, EV, and EV-depleted samples for normal (blue bars, non-MA), and overt (orange bars, overt MA status) patients. (b) qPCR results for miR-10a-5p, miR-200a-3p, miR-200c-3p, and miR-31c-5p in urine, EV, and EV-depleted samples for intermittent MA (purple bars), persistent MA (red bars), and persistent non-MA (yellow bars) patients. Values are represented as the inverse of ΔCt values (maximum number of cycles – ΔCt) to give the linear range, which is directly proportional to the concentration of each miRNA in each sample. Statistically significant (P ≤ 0.05) comparisons are indicated by an asterisk. EV, extracellular vesicle fraction; EV-dep, EV-depleted fraction; IMA, intermittent (microalbuminuria); MA, microalbuminuria; PMA, persistent (microalbuminuria); P (nonmicroalbuminuria), persistent (nonmicroalbuminuria).