FIGURE 4.

Activation of PLC-PKC-ERK 1-2-AKT-dependent transduction pathway in presence of SOD1^wt and SOD1^G93A. Western Blotting analysis of the levels of P-ERK 1-2 and P-AKT in SK-N-BE (A,B) and NSC-34 (C,D) cells incubated for 10 min with 400 ng/ml of SOD1^wt and with 400 ng/ml of SOD1^G93A in absence and in presence of Pirenzepine 10 μM for 5 min. The data represent the means ± SEM of three independent experiments relative to control obtained by densitometric analysis of P-ERK1/2 and P-AKT protein bands normalized to ERK1-2 and AKT respectively. ^∗p < 0.05 vs. Ctr; ^∗∗p < 0.01 vs. Ctr; ^∗∗∗p < 0.001 vs. Ctr; ^#p < 0.05 vs. SOD1^wt; ^##p < 0.001 vs. SOD1^wt; °p < 0.01 vs. SOD1^G93A.