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. 2018 May 24;9:611. doi: 10.3389/fphys.2018.00611

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Copyright © 2018 Damiano, Sasso, Accetta, Monda, De Luca, Pavone, Belfiore, Santillo and Mondola.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorimetric Determination of Reactive oxygen species (ROS) levels. SK-N-BE (A) and NSC-34 (B) semiconfluent cells were incubated for 18 h in medium containing 0.2% FBS and then for 30 min with 400 ng/ml SOD1^wt and mutated SOD1^G93A. To determine Reactive Oxygen Species (ROS) levels, SK-N-BE (A) and NSC-34 (B) cells were incubated with 10 μM of the ROS sensitive probe DCHF-DA and ROS levels were measured by fluorometric analysis. The graphs on the left show the mean ± SEM values (n = 6) relative to control of the indicated time points. The histograms on the right show the values of 30 min point and the relative ANOVA analysis. ^∗p < 0.05 vs. Ctr; ^∗∗p < 0.001 vs. Ctr; ^#p < 0.001 vs. SOD^wt.