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. 2018 May 24;9:611. doi: 10.3389/fphys.2018.00611

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Copyright © 2018 Damiano, Sasso, Accetta, Monda, De Luca, Pavone, Belfiore, Santillo and Mondola.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SK-N-BE and NSC-34 semiconfluent cells were preincubated with 15 μM of pirenzepine or with 10 μM of BAPTA-AM for 30 and 5 min respectively. Then, the cells were treated for 4 h with 400 ng/ ml of SOD1^wt or mutated SOD1^G93A. Induction of apoptosis by SOD1^G93A on SK-N-BE (A) and NSC-34 (B) cells is showed by Western Blotting analysis of cleaved form of PARP-1 protein bands. Evaluation of cell viability by trypan blue assay in SK-N-BE and NSC-34 cells are showed in (C,D), respectively; the data are reported as per cent variation compared to control. The data represent the means ± SEM relative to control obtained by densitometric analysis of cleaved PARP protein bands normalized to α-tubulin of three independent experiments. ^∗p < 0.005 vs. Ctr; ^#p < 0.005 vs. SOD^G93A.