FIG 2 .

SagS harbors a sensory HmsP domain flanked by two membrane-spanning regions that are similar to the HmsP domains of BifA and HmsP. (A) Overview of SagS domains. Vertical bars within the HmsP domain indicate transmembrane helices. (B) Localization of transmembrane helices present in SagS as determined using the TMHMM software for prediction of transmembrane helices in proteins (http://www.cbs.dtu.dk/services/TMHMM-2.0/). (C) Alignment of the sensory HmsP domain of SagS with the HmsP domains of BifA from P. aeruginosa and HmsP from Y. pestis. Residues subjected to site-directed mutagenesis are highlighted in boldface; cassette mutations are underlined. Predicted membrane-spanning regions are shown by bars below the alignments: black bars are based on TMHMM and PHYRE2, and gray bars are based on secondary structure prediction of HmsP by Y. pestis.