FIG 6 .
Susceptibility of ΔsagS mutant biofilm cells (2 days old) expressing sagS or sagSmut to tobramycin. Biofilms by the P. aeruginosa PAO1 wild type and ΔsagS mutant harboring the empty plasmid pMJT-1 were used as a control. Viability was determined via CFU counts. Susceptibility is expressed as log10 reduction. Experiments were carried out in triplicate. Error bars denote standard deviation. Biofilm susceptibility is ranked as ΔsagS like (significantly different from PAO1; P < 0.01), wild-type like, or intermediate (significantly different from PAO1 and the ΔsagS mutant, with log reductions ranging from 1.8 to 2.2; P < 0.05).