TABLE 2 .
Substitution | Restoration of ΔsagS biofilm-related phenotypes to wild-type levelsa |
|||||
---|---|---|---|---|---|---|
Attachment | Biofilm architecture |
BfiS phosphorylation |
Susceptibility to tobramycin |
Tolerance to tobramycin |
BrlR levels |
|
D105 | Yes | Yes | Yes | No | No | No |
L126 | Yes | Yes | Yes | No | No | No |
F131 | No | No | No | Yes | Yes | Yes |
L154 | No | No | No | Yes | Yes | Yes |
L141 | Yes | Yes | Yes | Intermediate | Yes | Yes |
R116 | Intermediate | Yes | Yes | Intermediate | No | No |
The wild type and ΔsagS mutant strain harboring the empty vector pMJT-1 or overexpressing sagS were used as controls. Data correspond to results shown in Fig. 3 to 7. “Yes” indicates restoration of ΔsagS biofilm-related phenotypes to wild-type levels, “No” indicates no restoration to wild-type levels, and “Intermediate” indicates values or phenotypes were in between those obtained for the PAO1 wild type or ΔsagS/pMJT-sagS and ΔsagS mutants.