Figure 2.

The DI activates macrophages (RAW264.7 cells). RAW264.7 cells are mouse macrophages cells and were incubated with different concentrations of herbal extract (0, 25, 50, or 100 μg/mL) for 24 h. The phagocytosis was determined by engulfing FITC conjugated beads, and was quantitated as the percentage of FITC-positive cells by using a flow cytometry. (a) A typical flow cytometric graph showing the amount of engulfed beads inside the cells in each treatment. (b) The data were presented as mean ± standard derivation (SD) of each treatment (P = 0.0288 compared to “0” control, one-way ANOVA, n = 6).