Figure 3.

The DI stimulates nitric oxide (NO) production by upregulation of NOS2 expression in macrophages. Protein was extracted from cultured RAW264.7 macrophages after 24 h of stimulation with 100 μg/mL of herbal extract (n = 3) or medium only (Control, n = 4). (a) Western blot analysis of NOS2 protein expression. (b) NO production in RAW264.7 macrophage cultures stimulated with different concentrations of herbal extract (0, 25, 50, or 100 μg/mL) for 24 h. The data were presented as mean ± SD of each treatment (P = 0.0284 compared to “0” control, one-way ANOVA, n = 7). NO production (over 10 μM) in LPS-stimulated cultures was not included.