Table 4.
Comparison of TM and ΔH° of transition of iM containing 5-hmC ± KCl (100 mM) and ±40% acetonitrile (ACN) at pH 6.1. The unmodified control iM VEGF sequence (iMWT) is also shown for comparison. Error bars are omitted for clarity and are <7% or 50% of the values shown for TM and ΔH°, respectively.
| iMWT | iMmod1 | iMmod2 | iMmod3 | iMmod4 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ΔTM (°C) | ΔH° (kcal/mol) | ΔTM (°C) | ΔH° (kcal/mol) | ΔTM (°C) | ΔH° (kcal/mol) | ΔTM (°C) | ΔH° (kcal/mol) | ΔTM (°C) | ΔH° (kcal/mol) | |
| Control | 52 | 16 | 52 | 17 | 53 | 15 | 47 | 12 | 51 | 16 |
| 100 mM KCl Alone | 48 | 18 | 49 | 8 | 48 | 7 | 44 | 13 | 49 | 7 |
| 40% ACN Alone | 50 | 20 | 48 | 17 | 50 | 18 | 46 | 12 | 49 | 16 |
| 100 mM KCl + 40% ACN | 40 | 10 | 40 | 9 | 41 | 10 | 40 | 5 | 42 | 12 |